How to Mix Semaglutide: Step-by-Step Reconstitution Guide: A Microdosing Perspective
Microdose Take
This guide originally addresses GLP-1 medication users on standard weekly protocols. The same underlying biology applies to microdosing, but the practical specifics differ. We've preserved the original guidance below; throughout, microdose-specific notes appear where the standard recommendations diverge.
⚠️ Educational only: GLP-1s are prescription medications. Microdose protocols are user-developed and not FDA-approved. Consult your prescribing healthcare provider before adjusting any dosing schedule.
What You'll Need to Mix Semaglutide
- Semaglutide vial — typically 2mg, 3mg, 5mg, or 10mg lyophilized (powder)
- Bacteriostatic water (BAC water) — contains 0.9% benzyl alcohol as preservative
- 3 mL reconstitution syringe with 21-23g, 1" needle for drawing BAC water
- 1 mL U100 insulin syringe (29-31g, 5/16" or 8mm) for weekly injections
- Alcohol swabs
- Sharps container
How Much BAC Water for Semaglutide?
For most users, 2 mL of bacteriostatic water is the standard amount because it produces clean syringe-unit math. Here's a quick reference:
| Vial Size | BAC Water | Concentration | 0.25mg dose | 0.5mg dose |
|---|---|---|---|---|
| 2 mg | 2 mL | 1.0 mg/mL | 25 units | 50 units |
| 3 mg | 2 mL | 1.5 mg/mL | 17 units | 33 units |
| 5 mg | 2 mL | 2.5 mg/mL | 10 units | 20 units |
| 10 mg | 2 mL | 5.0 mg/mL | 5 units | 10 units |
| 10 mg | 1 mL | 10.0 mg/mL | 2.5 units | 5 units |
💡 Try our free Reconstitution Calculator — enter your vial size, BAC water, and dose to see exactly how many syringe units to draw. Updates instantly with a visual syringe diagram.
Microdose Tools & Resources
Microdose Calculator
Split-dose math
Microdose Schedule
Daily/EOD cadences
Plasma Plotter
Compare microdose vs weekly
Standard-Dose View
GLP1Calculator article
Disclaimer: GLP1Microdose.com is independent and not affiliated with Novo Nordisk, Eli Lilly, or any pharmaceutical manufacturer. Microdosing protocols are not FDA-approved dosing regimens. This article is educational only and does not constitute medical advice.